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EMBO World Practical Course:
Structure determination of biological macromolecules by solution NMR
Beijing NMR Center, Peking University
Beijing, China
Mon September 8 - Mon September 15 2008
The intention of the course is to provide practical training in important aspects of structure determination by NMR. The emphasis will be on setting up experiments on the spectrometer (optimizing parameters, pulse programs), on processing and analyzing the NMR data and on structure calculations in a hands-on fashion. It is impossible to also teach all the necessary theoretical background of modern NMR experiments in a few days. Therefore, the course is aimed at PhD students and postdocs who have some working knowledge of NMR theory, i.e. product operator formalism, and who understand the basic principles of NMR pulse sequences. Possible candidates should have the level of knowledge of students that have participated in a past "Advanced NMR" EMBO lecture course on NMR theory organized by Boelens, Oschkinat and Griesinger.
Topics

Pulse sequences for assignment and structural information:
theory, practical set-up and use

Multidimensional data processing

Resonance assignment

Residual dipolar couplings: theory, experiments, data analysis

Relaxation: theory, experiments, data analysis

Structure calculation and evaluation