Our research mainly focuses on studies of
the structures, dynamics and biological functions of
proteins, especially enzymes. In particular, we are interested in the
correlation between protein dynamics (structural fluctuations over time) and
their functional mechanisms. We employ
Nuclear Magnetic Resonance (NMR) technique to determine
the solution structures and probe the dynamic properties of
bio-macromolecules. We also use biochemical methods to characterize the
activity and functions of proteins both in vitro and in vivo.
Our main goal is to elucidate the structural and dynamic basis of protein
Protein structures and dynamics
To provide structural basis for their biological functions, we use NMR technique to determine the solution structures of proteins and protein complexes. However, protein functions essentially depend on their motions, and static structures alone cannot fully explain their functions. NMR is a powerful tool to probe the motional properties of proteins. Therefore, we use relaxation phenomena, as well as newly developed NMR methods such as PRE and RDC to investigate protein dynamics and their correlation to functions.
Protein functions through interactions with other molecules (proteins, ligands, or nucleic acids). We use NMR methods to characterize protein interactions. In particular, we are interested in studying (1) interactions between enzymes and substrates during catalysis, (2) interactions between receptors and ligands in signal transduction pathways, (3) protein-protein interactions in the regulation of proteasome.
Membrane proteins play essential roles in cells. Recent advances in biochemical and NMR techniques have made it possible to use solution NMR method to study the structures and dynamics of membrane proteins. We are interested in studying the structures of protein cross-membrane translocation machineries, and to elucidate the molecular mechanism or the translocation processes.